Miracle Spore print until it probably got contamed - Mushroom Cultivation (2024)

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Miracle Spore print until it probably got contamed #18930751 - 10/04/13 11:21 AM (10 years, 9 months ago)

Ok so I ordered some special pscilocybe cyans from online and they came grounded into a powder. Long story short it was about 5.5 grams which me and my wife shared. A huge trip out of this world style later I go back to my moms to pick up our daughter. Driving around the neighborhood I find a freaking pan cyan growing in someone's yard. About 3 of them. I pick them and take them home do a clean sterile zip lock bag and covered with a sterile jar and took print. After print we ate the shrooms which did nothing after a 2.75 gram dried weight ps cyan trip, but point was I had what I wanted which was a grow able print by God's will. Kept print about 4 days now and bought all the supplies I needed..... UNTIL my daughter opened the bag last night and touched the wax paper that had the spore print. She had barely touched it and slightly tore the paper when I snatched it and put it bag in it's sterile environment. Now I'm afraid my print will be contaminated. I have everything so I really want to try to my syringe and then WBS colonize. If it's contamed could it still work or could I seperate the contam from good myc and get a real flush?

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The Final Cauliflower of Doom!

Registered: 02/22/13
Posts: 214
Loc: Upper Franconia
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Re: Miracle Spore print until it probably got contamed [Re: mushroomtexas] #18930839 - 10/04/13 11:45 AM (10 years, 9 months ago)

A print you´ve found outside is horribly non-sterile anyway. I never tried WBS, but with spore inoculation on rye grains and in rice cakes I usually had bad experiences. Nutrient-poor substrates like verm/bran are recommended for mushrooms that grow on wood, because many contaminants multiply on those less quickly than the mushroom hyphae. Verm/Sawdust (from broadleaf trees) maay also be worth a try.

This spring I just spilled my remaining spore solution over a heap of steamed woodchips (different broadleaf species), disposed them in a dark, moist valley, and a few months later a purely white and very rhizomorphic mycelium grew on them.

If I were you, I´d cut a piece off from that sporeprint and keep it for later, just for the case the sporesyringe-thing fails. Then you can still put them on agar. You don´t need petri dishes, you can use the same kind of glasses people use for PF-Tek, just with filter lids on them. If you´ve got some broad-spectrum antibiotics at home(children get ill quite often, just keep the meds), add a very small pinch of it to the agar when it cools down. Most first attempts with agar work fail, but even a small piece of a spore print contains millions of spores, its enough for lots of attempts.

Good luck, and when you succeed, put some infected pieces of wood in a nearby forest!

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Re: Miracle Spore print until it probably got contamed [Re: Rauhfasertapete] #18930875 - 10/04/13 11:51 AM (10 years, 9 months ago)

Sorry I need to clarify. The trip I had was ps cyan the wood based that I ordered dried up powder online. The spore print is actually pan cyan the dung growing gulf coast strain.
P.S. what I'm getting is go ahead and try to syringe with 2/3 prints and keep the last print in case the other 2 fail and if they do learn about agar and grow myc that way to seperate contams

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The Final Cauliflower of Doom!

Registered: 02/22/13
Posts: 214
Loc: Upper Franconia
Last seen: 8 years, 5 months

Re: Miracle Spore print until it probably got contamed [Re: mushroomtexas] #18930939 - 10/04/13 12:10 PM (10 years, 9 months ago)

Oh I overread that it was panaeolus.

Then another tip: Instead of inoculating all the spores directly into the substrate, inject some of them into a liquid culture solution, just sterilise water with 4% honey (also, add some antibiotics). Keep it in a glass or bottle with a tyvek filter lid for a month or so and see if it gets cloudy. LC colonised more quickly than spores, therefore its the safer method. And when the LC is almost used up, just inoculate it into another jar of nutrient solution. Once you´ve figured it out, its an endless supply.

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Re: Miracle Spore print until it probably got contamed [Re: Rauhfasertapete] #18935704 - 10/05/13 11:26 AM (10 years, 9 months ago)

Ok just read up about liquid cultures. Are they less prone to contamination vs WBS inoculation straight from syringe?

I'm thinking make 10 1 ml syringes as well as 3-4 jars of liquid culture. What kind of antibiotic can I use for the liquid culture that won't inhibit pan cyan growth and where can I get it?

1st time Pan cyan attempt and from an outdoor print my daughter touched. Bet it'll still work

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The Final Cauliflower of Doom!

Registered: 02/22/13
Posts: 214
Loc: Upper Franconia
Last seen: 8 years, 5 months

Re: Miracle Spore print until it probably got contamed [Re: mushroomtexas] #18935813 - 10/05/13 12:03 PM (10 years, 9 months ago)

The advantage of LC is that the mycelium is lively and happy in there, and starts to grow over the substrate immedately. In contrast, spores need some time to germinate, while the bacteria that may stick to them can start to multiply much faster, and thus prevail.

The good thing about antibiotics is that they´re only harmful to bacteria while mushrooms are usually tolerant. The tricky thing is that you can´t buy them as a private person (maybe in texas, in europe its controlled). I think you get them only if you or someone you know gets really sick. use whatever you get, but only in very small amounts, and do some research into the substance before, as specific antibiotics only kill certain bacteria. Most antibiotics are not autoclavable, so add them while the liquid is cooling down.

And antibiotics don´t kill bacteria immediately, they just block their metabolism; it may still take some time until they die. When the mushroom starts to grow in the LC, it produces its own antibiotics, that get dissolved in the liquid. The antibiotics you may add are just for the first time, before the mycelium has grown big enough to fight with its own weapons.

heck, i´m telling you to do all these horribly complicated things, although you still may be beginner... have you grown cubensis before? you´ve got to read a lot before you get started, because every mistake is a big mistake.

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Re: Miracle Spore print until it probably got contamed [Re: Rauhfasertapete] #18935885 - 10/05/13 12:29 PM (10 years, 9 months ago)

I've had a few successful cubes done cakes then chamber. I understand how to do liquid culture just wasn't sure if it'd help myc overtake bacteria spore easier. Even though it was a natural shroom I was confident it was relatively contam free until my daughter opened the bag. My other question is even if they do contam can't I take the contaminated mycelium and add manure for outdoor grow? sun kill bacteria but not fungus maybe?

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The Final Cauliflower of Doom!

Registered: 02/22/13
Posts: 214
Loc: Upper Franconia
Last seen: 8 years, 5 months

Re: Miracle Spore print until it probably got contamed [Re: mushroomtexas] #18936006 - 10/05/13 01:01 PM (10 years, 9 months ago)

I once had some P. cyanescens on a BRF-cake, which grew slowly and then got contamined by bacteria. I separated the more or less healthy mycelium from the really nasty stinky slimy stuff around, and lay it under a treetrunk in the forest. grew well from then on. It may perhaps work with panaeolus too, but it would be the last act of desparation.

I recommended you to use antibiotics because its really idiot-proof. nothing to worry about anymore, except perhaps molds. if you fail, you still have a print left to try again. and you´ll practically never use your print up, because you take only so few spores each time that you can barely see them. If you just keep trying, you´ll finally succeed with one method or another.

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